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糞便DNA提取試劑盒 FastDNA Spin Kit For Feces
貨號:116570200 品牌 MP Biomedicals
產(chǎn)品詳細(xì)描述
從人類和動物的糞便樣品中有效分離基因組DNA,直接用于PCR
1.多達(dá)500mg的糞便樣品
2.內(nèi)含配合FastPrep儀 器使用的理解介質(zhì)E
3.通常可以從500mg的糞便樣品中分離得到10-20μg總DNA
4.去除對下游實驗有影響的
快速高效提取糞便DNA,可裂解任何糞便樣品。可直接用于PCR等其下游實驗,The FastDNA? SPIN Kit for Feces Designed for use with the FastPrep? Instruments, host cells as well as bacteria, fungi, viruses, protists and other cells present in fecal samples are easily lysed within 40 seconds. These benchtop devices use a unique, optimized motion that provide an extremely quick, efficient and highly reproducible homogenization that surpasses traditional extraction methods.
Samples are placed into 2.0 mL tubes containing Lysing Matrix E, a mixture of ceramic and silica particles designed to efficiently lyse all stool organisms. Stool samples are pre-treated by washing in a Pre-Lysis Buffer. Homogenization takes place in the presence of MT Buffer and Sodium Phosphate Buffer. The pre-lysing and lysing reagents were carefully developed to protect and solubilize nucleic acids upon cell lysis, facilitate extraction of genomic DNA , and minimize RNA, humic acid or polyphenol contamination. Following lysis, samples are centrifuged to pellet debris and lysing matrix. DNA is purified from the supernatant with a silica-based GENECLEAN? procedure using SPIN filters. Eluted DNA is ready for digestion, electrophoresis, PCR and any other desired application.
產(chǎn)品應(yīng)用,如下:
The Fast DNA? SPIN Kit for Feces quickly and efficiently isolates PCR-ready genomic DNA from fresh or frozen human and animal stool samples.
產(chǎn)品屬性,如下:
Product Type Kits & Assays
Components Lysing Matrix E 50x 2.0 mL tubes, PLS Solution 25 mL, MT Buffer 8 mL, Sodium Phosphate Buffer 100 mL, PPS 25 mL, Binding Matrix 66 mL, Wash Buffer #1 120 mL, Wash Buffer #2 12 mL, TES 20 mL, BBS Gel Loading Dye 200 μl, SPIN Filter Tubes 50 each, Catch Tubes 50 each
Instrument Compatibility FastPrep-24; FastPrep 120; FastPrep-96; SuperFastPrep-1
Sample Type Soil; Feces; Environmental Water; Wastewater; Sludge
Sample Volume up to 500 mg per prep
Tube Compatibility 2 mL
Matrix Type E
Molecule Enriched DNA
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